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Bio-Organic Synthesis

Our Research Scope mainly employs the novel solvent called Deep Eutectic Solvent (DES). DESs are well known as ‘green’ liquid because their combinations are selected mainly from natural and non-toxic components. Studies on utilization of DES involved transformation of biomass resources to useful multiple purpose active agents (MPA).  The MPAs as a mean of reducing green house effects, could be applied in biological chemistry to liquid engineering. DES was studied to generate a safe and environmental friendly process for conversion of biomass to monosaccharide. DES was formulated by different ratios of tetrabutylammonium bromide  (TBABr) salt and will be applied for the enzymatic reactions replacing organic solvents in those enzymatic reactions. In this team, we synthesized a metallopeptide (4-8 amino acids) as catalyst for assymetric reactions and purified using HPLC-UV detector before allowing it to react with metal salt such as copper(II) acetate. Epoxides are industrially useful as intermediates and have wide range of applications in food, polymer and pharmaceutical formulations industries. An improved enzyme-facilitated epoxidation of a simple alkene (1-nonene) and lipase was used to catalyse the formation of peroxy acids instantly from H2O2 and various perhydrolysis substrates. The peroxy acid generated was then utilised directly for in-situ oxidation of 1-nonene to 1-nonene oxide. Highest conversion of 1-nonene was achieved using phenylacetic acid as an oxygen carrier with yield of 97% by Novozyme 435.